FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28661434
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1864910.6506687697482268No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG109900.029.136531
GCTCGGA52000.022.9982346
CGGAGAT58500.020.9376939
GGGCTCG57800.020.4399094
TCGGAGA58550.020.0537688
GGCTCGG60500.019.767115
CTCGGAG61950.019.6551257
CGTGGGC80000.015.3737421
TACACTG126250.014.8116345
TATACTG176400.014.0521315
CTACACT108300.012.7158194
GTGTAGG76300.012.2086691
CTATACT129350.012.1033764
GTGTAGA109750.011.6042311
TCTACAC119300.011.4238263
TACACAG172750.011.3701415
CTAGGAC58850.011.3327573
GTATTAG117300.011.1054941
ACAGTGC110100.011.0593568
TAGACTG103100.010.9668175
Produced by FastQC (version 0.11.5)