FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_9361.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_9361.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20963168
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG15200.028.616955145
AGACGGC64100.019.339884145
TACACTG189400.016.1153855
TATACTG266900.015.6734925
ATGCCGG71550.014.083836145
CTATACT161350.014.019234
CTACACT140650.013.8144494
ATACTGT333550.013.6501456
TCTCCGC62950.013.243959145
CACGAGC46600.013.068001145
GTATAGA162300.012.7304011
TCTATAC155900.012.649153
GTGTAGG95550.012.5948551
GTCCTAG99650.012.5131561
GTATTAG151600.012.3855791
GTATAGG81700.012.3341291
GTCTACG15950.012.2720981
CCTACAC93600.012.1608053
TATACAG221250.012.091545
TACACCG18650.012.0509535
Produced by FastQC (version 0.11.5)