FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_9109.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_9109.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19899604
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG125550.018.939848145
TAGATCG77800.018.170815145
TATACTG212850.015.5656935
TACACTG145300.014.7191365
GTATAGG77750.014.3595491
TCTCCGC132900.014.073888145
TACACCG57550.013.9831045
GTGTAGG91900.013.9629841
CTATACT133950.013.5849154
TACACAG178050.013.3147095
CTAGGAC75050.013.0409433
TCTATAC137550.012.8604213
CACGAGC181500.012.781819145
CTCCGAC61200.012.675111145
GTGTAAG107000.012.670051
GTATAGC77900.012.6567411
GTATAGA141050.012.6439411
CCTACAC91200.012.55995753
CTACACT116050.012.5567374
GTGTAGA127200.012.3678181
Produced by FastQC (version 0.11.5)