FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_8337.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_8337.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33201087
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC89800.026.883547145
TCTCCGC299650.020.395386145
CACGAGC246550.018.318998145
GAGCCCC374400.016.633207145
TATACTG327400.016.2537785
CTACACT190550.015.6756384
TACACTG261350.015.4792055
GTATAGA212200.014.6565321
GTATAGG120050.014.3725591
CTATACT216250.014.2149944
GTGTAAG188950.014.08116151
GTGTAGG140850.013.9486361
CCACGAC124900.013.87248145
CGTACAC34850.013.730243
CTAGGAC142050.013.7292873
TCTATAC209350.013.4714263
TAGACAG390150.013.1936125
CTTACAC192600.013.1749423
TATACAG284600.013.1447325
CCTACAC139450.013.10143853
Produced by FastQC (version 0.11.5)