FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_8337.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_8337.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19423774
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG211550.014.8389325
TACACTG149450.014.0194095
CCTATAC82700.013.9386073
AGACGCG36000.013.895112145
GTATAGG87750.013.7970761
GTGTAGG94150.013.1672011
CTATACT145950.012.9150784
GTACTAG60850.012.7479671
TATACCG38550.012.6002215
TACACAG182100.012.4214535
TACACCG47750.012.298145
GTATTAG153650.012.2203861
CTAGGAC66750.012.1645083
GTATAAG145050.012.09526351
ATACTGT260550.012.0482676
TCTATAC151900.011.9796383
GTGTAGA134300.011.9298221
TAGGACA117750.011.8829474
TAGACTG114600.011.8300015
TAGGACT109050.011.7007794
Produced by FastQC (version 0.11.5)