FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_8336.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_8336.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18719500
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC30700.020.308563145
CACGAGC80700.016.70931145
TCTCCGC102700.015.106451145
TATACTG221200.014.9128065
CTAGGAC79500.013.8614783
TACACTG163800.013.6765845
GTATAGG70550.013.6670171
GACTCCG82100.013.5991147
TAGGACT112400.013.3517164
GTGTAAG115050.012.8547221
GTATAGA124650.012.7952731
CTATACT137050.012.7488524
CTACACT115250.012.5812024
TACACCG15600.012.5479345
TACACAG202500.012.1010975
GTGTAGG80200.012.0225451
TAGACTG129850.011.8924325
ATACTGT284100.011.8918046
GTAGGAC54300.011.8829223
CCTACAC80750.011.8512583
Produced by FastQC (version 0.11.5)