FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_7651.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_7651.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19322112
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG179550.016.312665
TATACTG263850.014.865145
CTACACT126900.014.2825984
GTATAGG77400.013.8625051
GACCGGC31750.013.700215145
GTATAGA151200.013.4253991
TACACAG220250.013.29824355
GGATCGT27900.013.2524438
TCTACAC118300.013.1759693
CGTACAC20950.013.1500813
CTAGGAC89500.012.9606193
GTGTAGG90650.012.9559171
GTGTAGA123650.012.7229141
ATAGGAC85000.012.7085553
CTTACAC127900.012.6404683
ATACTGT323550.012.6152496
ACACTGT259700.012.4785596
CCTATAC77550.012.4336513
GTATTAG141800.012.2703231
TAGGACT119950.012.1485554
Produced by FastQC (version 0.11.5)