FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_7431.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_7431.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18466510
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG252750.016.1779215
TACACTG170050.014.3677275
TATACCG16000.014.0467725
GTCCTAG85500.013.9907081
GTATAGA146600.013.3521641
ATACTGT315100.013.2758466
GTATAGG75600.013.1377391
CTATACT149450.013.0009084
CCTATAC76800.012.6496463
TACACCG16550.012.2657715
CTACACT124050.012.15634
CCTACAC85850.011.9073093
TCTATAC139850.011.8715613
TAGACAG280150.011.7748555
TACCCTG110600.011.6680885
TAGACCT101350.011.6600034
GACTCCG76850.011.6036877
GTATTAG134600.011.5263191
TAGACTG131750.011.5008645
GGATCGT27200.011.4613128
Produced by FastQC (version 0.11.5)