FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_6751.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_6751.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23497699
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC60300.024.285873145
GAGACCG95200.024.216413145
ATGCCGG259700.022.165024145
TACATCG191450.018.895779145
TGCCGTG142800.016.905796145
TCTCCGC204750.016.889397145
CGTCTTG152350.016.417095145
GAGCCCC251850.016.09124145
CACGAGC319900.015.387753145
TACACTG190050.015.182565
TATACTG235850.015.1237345
CTACACT142100.014.3874844
GTGTAGG96900.014.0654831
GTATAGA158800.013.924191
GTATTAG152300.013.8996391
CTAGTAC72350.013.8283793
TACACAG230600.013.51882655
CTATACT157850.013.5030724
GACCTCG138900.013.204985145
TACACCG27100.013.1086095
Produced by FastQC (version 0.11.5)