FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_6215.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_6215.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21420257
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG287500.015.6346915
TACACTG182650.015.36125855
CACGAGC60900.015.2374735145
TACACCG26200.014.3892315
CTATACT170250.013.1585154
ATACTGT357850.012.7433946
TCTATAC160850.012.5753093
TATACAG215850.012.3939515
GTGTAGG99250.012.3446421
TCTCCGC85750.012.258975145
GTATAGA164900.012.134191
GTATAAG148050.011.9972031
CTTACAC139750.011.9838313
TACACAG231200.011.9473795
CTACACT140500.011.8682594
GGATCGT34350.011.6084298
ACTGTAC252400.011.6045838
CCTATAC88150.011.5966433
ACAGGCG202850.011.5799185
CTAATAC182200.011.5394513
Produced by FastQC (version 0.11.5)