FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_6215.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_6215.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21592020
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCGGC153750.019.332323145
GAGCCCC178800.016.745491145
TCTCCGC168700.015.9431505145
TACACTG144950.015.5552355
TATACTG218500.015.2298515
ATGCCGG297300.015.118617145
TACACCG70650.014.8795655
CTACACT126100.014.7183554
CTCCGAC75450.014.604937145
CTATACT154250.014.4763784
GTATAGA158150.013.706241
GTATTAG167300.013.04328251
TACACAG177550.013.0258015
TATACCG51000.012.9361685
CCTACAC105450.012.7879463
TCTACAC144450.012.748253
GGACCGT35800.012.5557616
CACGAGC245450.012.405137145
TAGACAG198900.012.3566095
GTATAGG85500.012.2947071
Produced by FastQC (version 0.11.5)