FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_6119.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_6119.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26447952
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG63000.035.44266145
CTCCGAC58150.023.188862145
AGACGGC294850.022.890999145
TCTCCGC196050.020.818897145
CACGAGC153600.020.059212145
ATGCCGG408050.018.335066145
CCACGAC87650.016.79037145
GAGCCCC253350.016.138918145
TATACTG296100.015.9393975
TGCCGTG256650.015.76192145
TACACTG222600.014.5583335
GTGTAGG110450.014.3089231
CTAGACT137600.014.0149814
CTACACT155500.013.9868584
GTATAGA177850.013.8592991
CCTACAC110650.013.4972453
CTTACAC165550.013.3567253
GCTAGAC113350.013.3036593
CTATACT183150.013.1815554
TACACAG270250.013.1449735
Produced by FastQC (version 0.11.5)