FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_4340.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_4340.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16868192
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG140750.016.637385
TACACCG94050.016.187935
CTACACT82900.014.9545164
TACACTG93700.014.7783045
TATACCG68600.014.5843065
TACCCTG97200.014.3953385
CTATACT122250.013.877074
GTATAGG81700.013.5766051
TACACAG114400.013.5618385
ACACCGT92400.013.4954486
TCTATAC130900.013.2923713
TGTACTG97050.013.0729435
TATACAG146500.013.0151425
GTATAGA136000.013.0068681
CCTACAC89800.012.9981153
GTGTAAG95750.012.7201541
GGACCGT34850.012.481866
TAGACAG122150.012.4046255
ATACTGT180500.012.2906556
GTATAAG130350.012.2358311
Produced by FastQC (version 0.11.5)