FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_4329.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_4329.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18127303
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG202200.016.2425385
TACACCG42800.013.890155
CCTACAC88250.013.6373583
TACACTG143850.013.6078885
GTATAGA131050.013.4980341
CTATACT128250.013.3976254
CTACACT108650.013.1453864
TCTCCGC99250.013.074955145
GTGTAGG87350.012.7813151
GTACTAG59000.012.7790641
CTAGGAC71100.012.6441313
GTATAAG118550.012.5974721
ATACTGT259750.012.4205656
TATACAG172950.012.3662585
GTATTAG133150.012.3050951
TCTATAC133550.012.2144943
GCTACAC78500.012.0986943
TAGACAG216200.012.07212455
TAGGACA107550.012.0664484
TACACAG176800.012.0559645
Produced by FastQC (version 0.11.5)