FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_341.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_341.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18147000
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC44650.019.808687145
ATGCCGG66250.018.602886145
CACGAGC67500.015.251125145
CTCCGAC27350.015.10897145
TATACTG214500.015.0067745
TACACTG160700.014.1208285
TCTCCGC69650.013.947649145
GTGTAGG84950.013.3130791
TACACAG196350.012.9970095
CTACACT114400.012.8647594
CTTACAC118600.012.837083
GTATAGA138350.012.6810011
GTATTAG134150.012.6456881
CCTACAC81800.012.5853913
CTGATCG51050.012.496956145
CTATACT139000.012.4134884
GTATAGG71200.012.2185191
GTACTAG60600.012.0827571
TAGACAG256000.011.8660385
ATACTGT270650.011.8130546
Produced by FastQC (version 0.11.5)