FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_3256.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_3256.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18880292
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG179100.015.9490795
TATACTG248950.015.609455
ATGCCGG75500.014.211294145
GTATAGG74950.013.6384921
GTGTAGG87850.013.616361
GTATAGA145000.013.1494211
CTATACT146250.012.9383724
TACACAG218500.012.8408725
CTACACT124750.012.6111354
GTCCTAG89250.012.5904821
CTAACAC145100.012.4413333
TAGACTG136550.012.370845
ACTGATC127950.012.35242758
ATACTGT311950.012.247876
GGACCGT14850.012.2053016
CCTACAC85350.012.1469563
TAGACAG283300.012.0533915
TCTCCGC59600.012.042254145
CTAGTAC57600.011.9573813
GTGTAAG129750.011.901211
Produced by FastQC (version 0.11.5)