FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30982.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30982.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16797593
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG84250.016.349925
TATACTG127600.015.3975135
TACACCG107950.014.3721945
CTATACT114550.013.6706954
GTGTAGG96500.013.0719671
GTATAGG82100.013.0688551
GTATAGA134500.013.0440391
CTACACT85100.012.7789054
CTAGACT47150.012.7622824
TACACAG96800.012.5824695
CCTACAC95600.012.51293
TAGACAG94700.012.4787065
TCTACAC117350.012.3560273
GTATAAG122750.012.28459551
GTGTAGA122700.012.2305171
TAGACTG62100.012.1415385
GTATTAG140200.012.1000421
CTACCCT61150.011.9744844
CTACACC156200.011.9284514
TATACAG147650.011.8826665
Produced by FastQC (version 0.11.5)