FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30978.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30978.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19736239
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG191150.016.3467865
TACACTG142200.014.8871895
GTATAGA161950.013.2057091
CTACACT118150.013.1313534
TACACCG68200.013.0752565
TAGATCG45700.013.008133145
CTATACT149050.012.74379354
GTATAGG92400.012.710531
ATACTGT253000.012.3792036
TAGGACT108050.012.3458924
CCTACAC103550.012.2522943
TATACAG193950.012.07375055
CTACACC140600.012.0143434
GTATTAG159000.011.9004681
TAGACTG109250.011.8784855
CTAGGAC65000.011.82284453
GTATAAG149450.011.7877511
TACACAG176650.011.7376695
TCTACAC136900.011.70356753
GTCTAGA96050.011.6991671
Produced by FastQC (version 0.11.5)