FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30976.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30976.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23269336
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG69250.025.439295145
CACGAGC146150.017.857565145
CTCCGAC64850.017.327675145
TCTCCGC177900.017.31935145
GAGCCCC188750.016.016502145
TATACTG243250.015.7069295
ATGCCGG350050.015.346408145
TACACCG55800.015.0715285
TACACTG166100.014.8839525
CTACACT132850.014.0796434
CTAGGAC89050.013.8404253
CCTATCG300150.013.574272145
GTATAGG88450.013.1961591
TGCCGTG224500.012.981602145
AGACGGC378150.012.959886145
CTATACT159100.012.9414124
GTATTAG161900.012.8515231
TAGACAG247150.012.8190065
TACACAG217050.012.5591885
CTAGACT105100.012.4855994
Produced by FastQC (version 0.11.5)