FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30908.TR4_batch_6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30908.TR4_batch_6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11644426
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG54050.026.423584145
CTCCGAC26100.021.665829145
TCTCCGC63950.018.81866145
CGAGACA46000.017.178682145
ATGATCG48500.017.040577145
TATACTG149300.016.7530365
TGCCGTG46450.016.387955145
CGTCTTG37200.016.370335145
CTACACT72800.014.7388834
GTACTAG37050.014.6755451
GTATTAG79400.014.5176991
GTAGGAC34650.014.4371043
CACGAGC54200.014.312177145
GTGTAGG51800.014.2755091
TACACTG102400.014.2308335
TACACAG121250.014.2308045
ATACTGT191200.013.9917726
GTCCTAG54350.013.8725081
GTCTAGG54550.013.6887461
TCTACAC74200.013.6791273
Produced by FastQC (version 0.11.5)