FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30904.TR3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30904.TR3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18751308
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG233500.016.704555
TACACTG154100.015.949115
CTACACT122300.015.0572824
ATACTGT294300.013.5984126
CTATACT148100.013.3643244
CCTACAC91700.012.8871743
GTATTAG138550.012.7150551
TCTATAC144350.012.4558753
GTATAGA142800.012.4381691
GTGTAGG92750.012.4280151
TACACCG38300.012.3042165
CTTACAC118800.012.14443
GTATAGG79250.012.0751691
TCTACAC123550.011.9709013
GTCCTAG82250.011.6347371
CTAGGAC76400.011.5772613
GTGTAAG117350.011.4907511
TATACAG189500.011.4776095
GTAGGAC58800.011.4668733
TACACAG198050.011.348185
Produced by FastQC (version 0.11.5)