FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30891.TR4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30891.TR4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21700488
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCCCC156050.016.724707145
TATACTG151500.015.8873795
TACACTG105050.015.5279185
TACACCG111200.014.2127635
CTATACT135950.014.1316694
ATGCCGG227100.014.014162145
CTACACT98800.013.5750544
CGAGACA115400.013.506799145
TATACCG70300.013.0971015
GTATAAG141800.013.0372121
GTATAGG96500.012.8466411
CCTATAC93700.012.6891363
GTGTAGG115250.012.6437491
TCTCCGC214350.012.581697145
TAGACAG116150.012.35875
TACACAG121700.012.3312355
TATACAG168950.012.3154555
GTCCTAG43650.012.2904631
GTATAGA147800.012.1646061
GTGTAGA138200.012.0129091
Produced by FastQC (version 0.11.5)