FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30890.TR5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30890.TR5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17594456
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG131900.015.3352135
TACACTG89650.014.4754865
TATACCG68150.014.0423075
TACACAG110450.013.9155555
GTATAGA151950.013.5021391
TAGACTG70600.013.0415555
CTACACT95700.013.0300744
CTATACT128100.012.960354
TACACCG101750.012.896585
GTGTAGG99550.012.6714061
CTAGGAC50350.012.6710793
TATACAG158750.012.5131675
GTATTAG158600.012.3874791
TAGGACT93700.012.3023464
TCTATAC141600.011.9807213
ACACTGT101950.011.9468236
TAGTACT96750.011.7646514
GTATAAG134550.011.745991
TAGACAG91750.011.6946175
CTTACAC94550.011.6550043
Produced by FastQC (version 0.11.5)