FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30889.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30889.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19697164
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG193850.015.7080595
TACACTG130600.014.6554725
TACACCG59900.013.7980315
GTATTAG158850.013.280791
CTTACAC112100.013.2582843
CTATACT147950.013.2308554
TATACCG50000.013.1950325
GTATAGG91600.013.1380441
CTACACT114150.013.0201824
TATACAG181650.012.5323735
TCTATAC153600.012.4609683
CTAGGAC78700.012.3443773
GTATAAG145700.012.1905881
GTATAGA157500.012.1518241
GTGTAGG86200.012.0267111
TAGGACT119500.011.8305734
TACACAG159700.011.8034115
TAGGACA108750.011.8000294
GTAGGAC65800.011.7895423
GTCCTAG78850.011.7686431
Produced by FastQC (version 0.11.5)