FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_30830.TR2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_30830.TR2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24512663
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC74950.018.668207145
TCTCCGC188300.016.439724145
TACACTG181600.015.9289565
GAGCCCC211450.015.565573145
TACACCG55650.015.5028685
TATACTG247500.015.2613435
CACGAGC150400.014.894558145
CTACACT143050.014.7480764
GTATAGG106400.014.3085461
CTATACT171700.014.2717314
TACACAG221100.014.2636545
GTGTAGG111600.013.7717621
CTAGACT103050.013.5077644
CCTATAC107000.013.3479193
GTGTAAG152300.012.8523251
TCTATAC179500.012.7630293
GTATAAG168650.012.7239881
TATACAG230000.012.6715135
CCTACAC117450.012.654123
CTTACAC148400.012.5553943
Produced by FastQC (version 0.11.5)