FastQCFastQC Report
Sun 4 Aug 2019
HVWLKBGXB_n01_MG_2397.TR1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWLKBGXB_n01_MG_2397.TR1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24456564
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG87400.022.810854145
AGACGGC287150.018.682854145
TCTCCGC208300.017.506487145
TACACCG71750.016.1672155
TATACTG235500.016.1316035
ATGCCGG385500.016.041498145
GAGCCCC203800.015.580816145
CTATACT165150.014.7940984
TACACTG166750.014.6956245
CACGAGC184900.014.507251145
CTCCGAC91600.013.771274145
CTACACT135450.013.6489234
GTGTAGG118800.013.6085071
TATACAG218700.013.5253525
GTATAGG100150.013.2471431
GTATAGA180700.013.2396911
GTGTAAG144750.013.1221441
GTATAAG162000.013.0674231
TAGACAG225750.012.8139295
TAGACTG131900.012.7520615
Produced by FastQC (version 0.11.5)