FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_63915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_63915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57333997
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6213101.0836676884746061No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT28600.031.179283145
AGAGCGG174950.028.303087145
CGTCGTT28850.026.888403145
GCGTCGG69300.023.85221145
CGGTGGG188950.019.76002145
TCTCGGG120550.017.199898145
CGGAAGC141050.016.807446145
TCGTGTT121350.016.309847145
TCGGAAC41450.015.04184145
CGCCGTG36500.014.499633145
CCGTATA69250.013.819146145
TCGCCGG148500.013.620868145
GGGGGAA1803650.011.04687450-54
GCCGTAA57950.010.258585145
GGGGGTA457200.010.11079750-54
CGTATCG10155.677737E-69.28548145
AAAGGGG3673350.08.64071565-69
AAAAGGG3873400.08.59874165-69
GGGGTAA417800.07.944209650-54
GCCGTAG46100.07.5486164145
Produced by FastQC (version 0.11.2)