FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_63744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_63744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43016517
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5055081.1751486062899978No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT20850.044.16034145
CGTCGTT23200.042.187183145
AGAGCGG143550.035.80781145
GCGTCGG56850.032.264492145
CGCCGTG20250.026.135609145
CGGTGGG146550.024.339634145
CGGAAGC110350.023.78324145
TCTCGGG97400.023.000341145
TCGTGTT97900.022.5126145
TCGGAAC32850.021.849152145
CCGTATA54750.015.890292145
TCGCCGG118450.015.056874145
GCCGTAA41800.013.008276145
GGGGGAA1314150.011.43228450-54
GGGGGTA353500.011.20643750-54
CGTATCG6707.4063995E-510.820815145
GCCGTAG32300.010.549457145
CGGAAGG70200.010.121007145
TCGGAAA61450.09.320517145
AAAGGGG2689800.08.97779665-69
Produced by FastQC (version 0.11.2)