FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_58619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_58619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60687031
Sequences flagged as poor quality0
Sequence length151
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5446420.8974602827414642No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTG10100.040.91472145
TCTCGGG46600.028.003527145
CGGTGGG95300.026.169271145
CGCCGTT38100.025.688272145
AGAGCGG136500.023.316214145
GCGTCGG36950.020.405394145
CGTCGTT82700.019.286049145
TCGCCGG56100.017.316816145
GAGCGTG58650.015.822245145
CGGAAGC46250.014.891485145
GGGGGAA1454400.012.93177450-54
TCGTGTT231400.012.84537145
GATCGGG27250.012.770293145
CGGAAGG77000.012.051618145
GGGAGAG372650.011.68457220-24
AAAGGGG2531800.011.10859265-69
GAGAGGG370600.011.0918925-29
GGAGAGG366500.010.88366720-24
GGGGAGC45400.010.8561996
GAGTGTT209900.010.810804145
Produced by FastQC (version 0.11.2)