FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_53788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_53788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52872953
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3644580.6893089553745939No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTT32050.041.848186145
CGCCGTT31200.039.967484145
GCGTCGG84000.035.904343145
AGAGCGG244750.034.687035145
TCGGAAC51450.027.336903145
CGGTGGG221100.026.002638145
CCGTATA70850.024.047005145
TCTCGGG146600.023.094889145
CGGAAGC197400.022.954393145
TCGTGTT129450.018.929867145
TCGCCGG163900.018.357016145
CGCCGTG32450.017.650026145
GCCGTAA72500.016.799805145
TCGGAAA91450.013.080767145
GGGGGAA1516500.010.18404750-54
GTAGATA519500.09.992184145
GCCGTAG50100.09.695496145
AGCGTCC67450.08.92132145
GGAAGAC505500.08.719978145
TCGCCGA29550.08.341697145
Produced by FastQC (version 0.11.2)