FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_53652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_53652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68885512
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3829010.5558512797291831No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT20050.029.650642145
CGTCGTT23700.026.307812145
AGAGCGG162150.021.819132145
GCGTCGG49950.021.33617145
CGGTGGG174450.015.792369145
TCGCCGG100500.015.149136145
CGGAAGC122750.014.411294145
TCTCGGG115900.013.198776145
TCGGAAC42000.012.77371145
CCGTATA77600.012.6126585145
CGCCGTG26300.011.02653145
GCCGTAA64700.010.196984145
TCGTGTT123500.09.803568145
GGGGGAA1565600.08.41423650-54
GCCGTAG43500.07.4999423145
GTCGCCC82100.07.1528063145
ACCGCGC44850.07.11336951
GGGGGTA406250.06.19622150-54
TCGCCGA19901.5013007E-56.193419145
AAAGGGG3174950.05.970117665-69
Produced by FastQC (version 0.11.2)