FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n02_12976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n02_12976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66140393
Sequences flagged as poor quality0
Sequence length151
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3639130.5502129387105396No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTT33250.028.563526145
AGAGCGG156550.026.582205145
GCGTCGG72500.019.199741145
CGGTGGG177150.018.620975145
TCTCGGG120700.016.878399145
CGTCGTT32300.016.834139145
CGGAAGC121150.016.336966145
CGCCGTG42450.016.053965145
TCGCCGG154550.012.384176145
TCGTGTT134550.012.0697145
TCGGAAC43250.011.231063145
GCCGTAA57550.08.8183145
GGGGGAA1256450.07.74609950-54
CCGTATA76850.07.169715145
GCCGTAG51300.07.066182145
CGTATCG13702.4026074E-46.8794694145
CGGAAGG99200.06.796784145
GGGGGTA397450.06.457539650-54
GGGGGGA1896750.05.940775450-54
GAGCGTG74550.05.7376833145
Produced by FastQC (version 0.11.2)