FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_63915.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_63915.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57333997
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC13466072.348705951897964TruSeq Adapter, Index 2 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1220260.21283358283916606No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1153550.20119825240860148No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG170450.025.749527145
TGTATCG228300.025.452913145
CACGTCG35000.023.421854145
GAGCACA9457700.022.1884379
GTCTTCG94300.022.156015145
AGAGCAC9647300.021.7502048
CTGAACG83650.021.334381145
CGGAAGA10053850.020.8203434
GAAGAGC10107150.020.7881436
AAGAGCA10216600.020.5813227
TCGGAAG10259400.020.3846843
ATCGGAA10444200.020.043342
GATCGGA10573400.019.6910931
GGAAGAG10695950.019.668865
CGGAAGC146900.018.519121145
CACACGG109650.016.275612145
GAGCACC327500.014.77493145
AGGGGGG3035800.013.44761570-74
AAGGGGG3034400.013.42753970-74
AAAGGGG3340700.012.28363770-74
Produced by FastQC (version 0.11.2)