FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_63744.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_63744.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43016517
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGC10330412.401498475573929TruSeq Adapter, Index 3 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1789670.41604251687787736No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1300940.30242801852135076No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCATCG137650.038.36677145
ATGCCGG91100.035.276497145
CACGTCG24150.033.643486145
CTGAACG66550.030.521797145
CGGAAGC117100.025.337662145
GTCTTCG63550.023.743673145
CACACGG80650.021.677677145
GAGCACC245400.021.136429145
GAGCACA8569400.018.6711799
AGAGCAC8774550.018.2354938
AAGAGCA9232650.017.3747087
GAAGAGC9221450.017.3590416
CGGAAGA9312250.017.153074
TCGGAAG9541450.016.7350063
GGAAGAG9708600.016.5066285
ATCGGAA9731400.016.4098052
GATCGGA9877700.016.0587621
TCGGAAC34550.015.117659145
CATCGGA40350.013.8377971
AAGGGGG2242600.013.82779570-74
Produced by FastQC (version 0.11.2)