FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_58619.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_58619.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60687031
Sequences flagged as poor quality0
Sequence length151
%GC23

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC12052781.9860553072698512TruSeq Adapter, Index 4 (100% over 50bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1898650.31285926642217843No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1663100.27404537223117736No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG72800.031.190937145
CCAGTCC45950.030.313154145
TCACTGC154650.029.224937145
GAGCACA6813500.027.1590929
CCAATCG98050.027.006027145
AGAGCAC6960650.026.5829248
GAAGAGC7348150.025.212866
AAGAGCA7398450.025.0607017
GAGCACC113600.024.90587145
CGGAAGA7564250.024.4702174
GGAAGAG7637050.024.2628655
TCGGAAG7712800.024.0016753
ATCGGAA7931650.023.3598122
GATCGGA7915550.023.2255761
TGCCGTG28850.018.356611145
AAGGGGG2036700.017.7369970-74
CGGAAGC76400.017.661802145
AGTCACG51000.017.638716145
GCCGTCG26900.017.529783145
CTGAACG98850.016.732992145
Produced by FastQC (version 0.11.2)