FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_53788.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_53788.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52872953
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC9619851.8194274112134423TruSeq Adapter, Index 1 (100% over 50bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG945020.17873410626412337No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG107750.074.66467145
CACGTCG45600.041.999096145
ATGCCGG123800.038.55726145
CTGAACG110000.037.195236145
ACGAGCG37600.028.940384145
CGGAAGC244300.025.715519145
GAGCACC392000.025.353352145
GTCTTCG71550.025.144497145
CACACGG160500.024.362093145
ACGCTCG28850.022.379227145
CACGATG201400.018.622242145
GCCGTCG45150.017.031347145
TCGGAAC62750.016.185093145
TCACGCG59550.016.08026145
AAGAGCC532650.015.648726145
ATCACGC456250.015.311731145
TCACGAG625700.014.307044145
CCAGTCC478600.010.382901145
GAGCACA14534500.010.147459
AGAGCAC14948500.09.8635818
Produced by FastQC (version 0.11.2)