FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_53652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_53652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68885512
Sequences flagged as poor quality0
Sequence length151
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGC7695501.1171434713296462TruSeq Adapter, Index 5 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGATCG138500.034.77877145
ATGCCGG114150.029.614603145
CACGTCG24650.023.249096145
CGGAAGC141400.017.853596145
GTCTTCG85200.016.773455145
CTGAACG86500.016.269773145
GAGCACA7958850.015.2313999
AGAGCAC8098450.014.954568
CGGAAGA8288050.014.5604794
GAAGAGC8498050.014.2870236
TCGGAAG8450150.014.2811963
ATCGGAA8605850.014.0297362
AAGAGCA8751450.013.9388567
GATCGGA8694100.013.7846461
GGAAGAG9163800.013.2846245
GCCGTCG61500.012.149488145
CACACGG123650.012.085622145
GAGCACC370800.010.916676145
AGGGGGG2309350.010.30773770-74
AAGGGGG2399500.09.94947670-74
Produced by FastQC (version 0.11.2)