FastQCFastQC Report
Mon 5 Dec 2016
HVWJNBGXY_n01_12976.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVWJNBGXY_n01_12976.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66140393
Sequences flagged as poor quality0
Sequence length151
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGC6705751.0138660651744238TruSeq Adapter, Index 6 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG92450.024.403605145
CGGAAGC125050.015.779226145
CTGAACG88500.014.918618145
CACACGG100450.014.227113145
GAGCACA7238900.014.0710479
AGAGCAC7364200.013.8228248
GTCTTCG77050.013.746138145
CGGAAGA7592750.013.3690234
GAAGAGC7744750.013.2013276
AATATCG217450.013.110934145
TCGGAAG7744000.013.1079393
ATCGGAA7899400.012.8709012
AAGAGCA7970300.012.867957
GATCGGA7961650.012.7246411
GGAAGAG8333050.012.3197975
CACGTCG35700.011.989037145
GAGCACC318700.010.789387145
AGGGGGG2004700.09.96482870-74
AAGGGGG2106300.09.50481270-74
GCCGTCG62450.08.363741145
Produced by FastQC (version 0.11.2)