FastQCFastQC Report
Thu 25 Aug 2022
HVVYVAFX3_n02_dad4_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVVYVAFX3_n02_dad4_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6055322
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC273820.45219725722265475Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC158140.2611586964326587Illumina Single End PCR Primer 1 (100% over 49bp)
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC98810.16317877067478823Illumina Single End PCR Primer 1 (100% over 49bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)