FastQCFastQC Report
Thu 25 Aug 2022
HVVYVAFX3_n02_dad4_13.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVVYVAFX3_n02_dad4_13.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8542598
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC200390.23457734988817217Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC157400.18425308085432557Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG105620.12363920203198138No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG98250.11501185002501581Illumina Single End PCR Primer 1 (100% over 32bp)
TCGTGTAGGGAAAGAGTGTAGATCGGAAGAGCGTCGTGTAGGGAAAGAGT95140.11137127136264635Illumina Single End PCR Primer 1 (100% over 31bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)