FastQCFastQC Report
Thu 25 Aug 2022
HVVYVAFX3_n02_dad4_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVVYVAFX3_n02_dad4_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11192832
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCC774770.692201937811628Illumina Single End PCR Primer 1 (100% over 50bp)
CAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC770170.6880921646996935Illumina Single End PCR Primer 1 (100% over 49bp)
GAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC359360.32106262293582183Illumina Single End PCR Primer 1 (100% over 49bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG247240.22089137047710533No Hit
TAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGC208310.18611018194501625Illumina Single End PCR Primer 1 (100% over 49bp)
TGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG128670.11495750137230684Illumina Single End PCR Primer 1 (100% over 48bp)
CGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCG127180.11362629225561502Illumina Single End PCR Primer 1 (100% over 48bp)
CGCTAAATAAGGACATGACACATGCGAATCATACGTTTATTATTGATAAG121730.10875710454691001No Hit
TCGTGTAGGGAAAGAGTGAGATCGGAAGAGCGTCGTGTAGGGAAAGAGTG115030.10277113066648369Illumina Single End PCR Primer 1 (100% over 32bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)