FastQCFastQC Report
Thu 25 Aug 2022
HVVYVAFX3_n01_dad4_5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVVYVAFX3_n01_dad4_5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6055322
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTA308060.5087425573734973TruSeq Adapter, Index 16 (97% over 40bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGT179070.2957233323017339TruSeq Adapter, Index 16 (97% over 40bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGT108760.17961059709128596TruSeq Adapter, Index 16 (97% over 40bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGT65370.10795462239662895TruSeq Adapter, Index 16 (97% over 40bp)

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)