FastQCFastQC Report
Thu 25 Aug 2022
HVVYVAFX3_n01_dad4_12.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVVYVAFX3_n01_dad4_12.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11192832
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGTA952880.8513305658478569TruSeq Adapter, Index 7 (97% over 36bp)
GAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGT941200.8408953158592927TruSeq Adapter, Index 7 (97% over 36bp)
CAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGT449790.40185540174282974TruSeq Adapter, Index 7 (97% over 36bp)
AAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGT261170.23333683557476784TruSeq Adapter, Index 7 (97% over 36bp)
CAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCG164900.14732643177347787TruSeq Adapter, Index 7 (97% over 36bp)
CGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCG160610.14349362163213028TruSeq Adapter, Index 7 (97% over 36bp)
CACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGTC145480.1299760418096153Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
ACACTCTTTCCCTACACGAAGATCGGAAGAGCACACGTCTGAACTCCAGT140320.12536594849274965Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
TAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCGT132870.11870990290929052TruSeq Adapter, Index 7 (97% over 36bp)
TAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCG120340.1075152383239559TruSeq Adapter, Index 7 (97% over 36bp)
TGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATCTCG119480.10674688943781163TruSeq Adapter, Index 7 (97% over 36bp)
CAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATC118400.10578198618544439TruSeq Adapter, Index 7 (97% over 36bp)
TCAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATATC115400.1031016993733132TruSeq Adapter, Index 7 (97% over 36bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)