FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29686441
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG18018946.069754201926732No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG6988862.354226294758607No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT4972281.6749330106630163No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC4950731.6676738043472437No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGA1791680.6035347921968821No Hit
ATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGACGC1755250.5912631965549525No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT1407870.47424681186943224No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA1315690.4431955989604817No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG1228330.4137680229165901No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC993370.33462077855678285No Hit
CGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGAC821660.2767795573743582No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN677710.22828940660148517No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTGACGCT407250.1371838409326332No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTG374580.1261788167870982No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAT331070.11152229396578726No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTT323880.10910031283305399No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTAA320650.10801227402099162No Hit
TCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTA318440.1072678264127384No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG893250.073.182521
TAGTGGA1852400.063.77090574
TAGTGGG127900.060.47231774
TAGTGGC126500.057.22149374
TCTCGGT84200.049.40092574
GATCTCG292750.049.2522173
AACTCGA45400.042.63111574
CGGGGTT47300.042.5395314
ATAGTGG3101300.042.4168573
CGATCGA1541150.042.2497672
TAGTAGA45550.041.84077574
ATAGTAG47600.041.5181573
TAGTCTG67200.039.8152974
CGGGTTT49800.039.58701714
GGTGTAG294800.037.53251367
AGGTGTA295950.037.418666
GAGGTGT295450.037.37388265
GAGAGGT296450.037.3132863
GGATAGT343550.037.23133566
AGAGGTG297550.037.16695864