FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_TRE_S5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_TRE_S5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8183029
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGTCTC829301.0134389111904651No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG447520.5468879555480006No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTGTCTC324970.3971267852038652No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTGTCT186490.22789849577705273No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGAGAA91640.11198787148377454No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCCTGTC84950.10381241469387435No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT7725250.073.6866151
CGACTCG7729900.073.553223
GACTCGG7742150.073.48814
ACTCGGT7753750.073.4025
CTCGGTG7750000.073.296
TCGGTGC7721150.073.213287
CGGTGCC7698150.073.1525658
GGTGCCA7733450.072.991839
CCGACTC7807200.072.9040152
GTGCCAC7749050.072.8378210
GCCACTT7738100.072.82522612
CCACTTT7740600.072.7965513
CACTTTT7782750.072.5848114
TGCCACT7768350.072.5825711
ACTTTTT7741100.072.5257615
CTTTTTC7729000.072.4104516
TTTTTCA7737000.072.2858317
CAACTAG7817650.071.87141422
TCAACTA7812900.071.8601821
AACTAGT7819600.071.8596523
Produced by FastQC (version 0.11.5)