FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_TRE_S4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_TRE_S4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10064908
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTTGTCTC1470841.461354639307185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG682380.677979371495497No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCTGTCTC358130.35582044068361085No Hit
TCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTCTGTCT246710.24511898171349403No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGACT9372350.073.766511
GACTCGG9419900.073.349074
CGACTCG9425850.073.3359153
ACTCGGT9446950.073.1338965
CTCGGTG9453300.072.947746
CCGACTC9487900.072.899522
TCGGTGC9417700.072.850347
CGGTGCC9400850.072.736048
GGTGCCA9446650.072.553539
GTGCCAC9494200.072.29710
GCCACTT9485650.072.2428712
CCACTTT9493300.072.1808213
TGCCACT9513200.072.06937411
ACTTTTT9486000.071.9782115
CACTTTT9548600.071.95109614
CTTTTTC9478500.071.7931716
TTTTTCA9477950.071.7352917
CAACTAG9570250.071.3798422
TCAACTA9569950.071.3484721
AACTAGT9580000.071.3273423