FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_TRE_S3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_TRE_S3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9505537
Sequences flagged as poor quality0
Sequence length80
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG740919077.94604344815028No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG795890.8372909389548427No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTATTTCCGCTTACACAG673420.7084502432634789No Hit
ACGATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACAGC447780.47107280735428203No Hit
ACGATCGATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC351070.3693321061187811No Hit
ACGATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG329940.3471029569397289No Hit
AGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC291840.30702105520182604No Hit
ACATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC282340.29702688022780827No Hit
ACGATGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC280060.29462827823404397No Hit
ACGATCATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC235420.24766617604034366No Hit
ACGATCGACCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG227260.23908170574687154No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC207000.21776781259175573No Hit
ACGATCGATCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC187760.19752697822332396No Hit
ACGATAGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG175850.1849974388611606No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTACACAG172850.1818413836062076No Hit
ACGATCGATCCACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC172250.18121017255521701No Hit
ACGATCGATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTACACAGC166860.17553979328048486No Hit
ACGATCGATACGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG161080.16945912682260877No Hit
ACGTCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC157780.16598746604216047No Hit
ACGATCGATCCGACTGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC147220.15487815154472598No Hit
ACGATCGTCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC145430.15299503857593735No Hit
ACGATCGATCTGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG127600.1342375501773335No Hit
ATGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG125260.13177582707847016No Hit
ACGATCGATCCGACTCGGTACCACTTTTTCAACTAGTTCCGCTTACACAG120800.1270838249327734No Hit
ACGATCGATCCGACTCGGTTCCACTTTTTCAACTAGTTCCGCTTACACAG120030.12627377075066878No Hit
ACGATCGATCCGACTCGATGCCACTTTTTCAACTAGTTCCGCTTACACAG118440.12460106146554371No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACGG117940.12407505225638489No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACGCAG117660.12378048709925595No Hit
ACGATCGATCCGACTCGGAGCCACTTTTTCAACTAGTTCCGCTTACACAG113680.11959345379435164No Hit
ACGATCGATCCGACTCAGTGCCACTTTTTCAACTAGTTCCGCTTACACAG105170.11064077705446836No Hit
ACGACGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC104660.11010424766112635No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGCTCCGCTTACACAG104010.10942043568921989No Hit
ACGATCGATCCGATCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC103140.10850517966528352No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTAAACTAGTTCCGCTTACACAG102810.10815801358723869No Hit
ACGATTGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAG98440.1035606930991905No Hit
ACGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCCTACACAG98310.1034239307048092No Hit
ACGATCGATCCGACCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC96060.10105688926359446No Hit
ACGATCGATCCGACTCGGTGCCATTTTTCAACTAGTTCCGCTTACACAGC95640.10061504152790106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG8715400.073.992021
GATCGAT8724950.073.360773
CGATCGA8763550.073.352652
ATCGATC8769650.073.041314
TCGATCC8643400.072.853755
CGATCCG8625200.072.727766
GATCCGA8686300.072.387837
ATCCGAC8740800.072.073988
TCCGACT8759850.071.741349
CGACTCG8794850.071.58658611
ATTTCCG156750.071.543835
GACTCGG8832600.071.5415912
ACTCGGT8826100.071.4000413
CTCGGTG8786550.071.27724514
TCGGTGC8777050.071.16405515
CCGACTC8862300.071.1064510
CGGTGCC8763900.071.0445616
GGTGCCA8753400.070.88718417
GTGCCAC8769900.070.71500418
GCCACTT8774450.070.7072520
Produced by FastQC (version 0.11.5)