FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_TRE_S2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_TRE_S2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6929651
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC553016579.80437975880747No Hit
CGATCGATCCGACTCGGTTCCACTTTTTCAACTAGTTCCGCTTACACAGC544300.7854652420446571No Hit
CGATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACAGCT322260.465045065040072No Hit
CGATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC304880.4399644368814533No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG288630.4165144824753801No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGATCCGCTTACACAGC168020.24246531318821107No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT157900.2278614031211673No Hit
CGATCGATCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT155110.22383522633390918No Hit
CGATCGACCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC146480.21138149670163767No Hit
CATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT136490.19696518626984247No Hit
CGATGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT130290.18801812674260218No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTACACAGC128130.18490108664924107No Hit
CGATCGATACGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC125600.18125010913248013No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACGGC117620.16973437767645153No Hit
CGATCGATCCGACTCGGTGCACTTTTTCAACTAGTTCCGCTTACACAGCT107050.15448108425662418No Hit
CGATCATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT103740.14970450892837173No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGT102340.14768420516415617No Hit
CGATCGATCCGACTCGGTGCCACTTTTTAAACTAGTTCCGCTTACACAGC94220.13596644333170602No Hit
CGATAGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC93610.13508616812015498No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACGCAGC88220.12730799862792513No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCAGCTTACACAGC82890.11961641358273309No Hit
TGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC81540.1176682635243824No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGCTCCGCTTACACAGC79910.1153160527131886No Hit
CGATCGATCCGACTCGGTACCACTTTTTCAACTAGTTCCGCTTACACAGC79350.11450793120750236No Hit
CGATCGATCCGACTCGATGCCACTTTTTCAACTAGTTCCGCTTACACAGC77840.11232888929038419No Hit
CGATCGATCTGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC76520.11042403145555238No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCCTACACAGC74810.10795637471497482No Hit
CGATCGATCCGACTCGGTGCCCCTTTTTCAACTAGTTCCGCTTACACAGC73630.10625354725656458No Hit
CGATCGATCCGACTCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT73580.10618139355069973No Hit
CGATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTACACAGCT72270.10429096645704092No Hit
CGATTGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGC72090.10403121311592749No Hit
CGATCGATCCGACTCGGTGCCATTTTTCAACTAGTTCCGCTTACACAGCT69370.10010605151688015No Hit
CGATCGATCCGACTGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT69360.10009162077570717No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCGA6515050.073.702771
GATCGAT6512100.073.645112
ATCGATC6525100.073.505293
TCGATCC6449550.073.3996054
CGATCCG6445800.073.34565
GATCCGA6486800.073.0965046
ATCCGAC6517750.072.9293067
TCCGACT6508150.072.698828
CGACTCG6505100.072.63634510
GACTCGG6523000.072.5805311
ACTCGGT6526950.072.5071712
CTCGGTG6401950.072.3812713
TCGGTGC6402750.072.3427614
CGGTGCC6390950.072.3097815
GGTGCCA6378400.072.2287416
CCGACTC6561200.072.12139
GCCACTT6394600.072.0877619
CCACTTT6506050.072.08687620
GTGCCAC6393600.072.0554117
CACTTTT6535800.071.896321
Produced by FastQC (version 0.11.5)