FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_TRE_S1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_TRE_S1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979763
Sequences flagged as poor quality0
Sequence length80
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT707571578.79623326361731No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG694920.77387343073531No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCC518910.5778660305400043No Hit
GATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT455600.5073630562410166No Hit
GATCGACCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT252640.2813437281139825No Hit
GATCGATACGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT204760.228023835372938No Hit
GATCGATCCGACTCGGTGCCCCTTTTTCAACTAGTTCCGCTTACACAGCT198150.22066283931992414No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTACGCTTACACAGCT195420.21762266999696983No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGTT188830.21028394624668825No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGATCCGCTTACACAGCT152380.16969267451713368No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTACCGCTTACACAGCT134680.1499816865990784No Hit
GATCGATCTGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT125020.13922416437939397No Hit
GATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACAGCTT122250.13613945045097517No Hit
GATAGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT117990.13139544996900251No Hit
GATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACAGCTA117620.1309834123684556No Hit
GATCGATCCGACTCGGTGCCACTTTTTAAACTAGTTCCGCTTACACAGCT117050.13034865174058602No Hit
GATCGATCCGAATCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT110750.12333287637992228No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACGGCT106260.11833274441652858No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCA105670.11767571148592673No Hit
GATCGATCCGACTCGGTACCACTTTTTCAACTAGTTCCGCTTACACAGCT105530.11751980536680087No Hit
GATCGATCCGACTCGATGCCACTTTTTCAACTAGTTCCGCTTACACAGCT103870.1156712042400228No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGG102770.11444622758974819No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACGCAGCT100770.1122189973165216No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGCTCCGCTTACACAGCT99410.11070448073072753No Hit
GATTGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT99310.11059311921706619No Hit
GATCGATCCGACTCGGTGCAACTTTTTCAACTAGTTCCGCTTACACAGCT97500.10857747581979613No Hit
GATCGATCCGACTCGGTGCCACTTTTCAACTAGTTCCGCTTACACAGCTG94410.10513640504766104No Hit
GATCGATCCGACTCAGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCT93420.10403392606241389No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCCTACACAGCT91210.10157283661049851No Hit
GATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCAGCTTACACAGCT90270.10052603838208202No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAT8336050.073.935631
ATCGATC8344850.073.605432
TCGATCC8227600.073.4709553
CGATCCG8213750.073.37984
GATCCGA8262650.073.0485465
ATCCGAC8268500.072.8138966
TCCGACT8284000.072.552637
CGACTCG8323100.072.405389
GACTCGG8369200.072.3956110
ACTCGGT8383000.072.2132811
CTCGGTG8363050.072.12453512
TCGGTGC8389000.072.0130913
CGGTGCC8359500.071.9274114
CCGACTC8374700.071.860468
GGTGCCA8323400.071.7543415
GTGCCAC8352350.071.571216
GCCACTT8336450.071.56373618
CCACTTT8370650.071.5125819
TGCCACT8366100.071.42747517
ACTTTTT8359800.071.3069821
Produced by FastQC (version 0.11.5)