FastQCFastQC Report
Fri 11 Oct 2019
HVV32AFXY_n02_CMV_S6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHVV32AFXY_n02_CMV_S6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8904815
Sequences flagged as poor quality0
Sequence length80
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT205472523.0743142895164No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA190073821.34505882491663No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG152764517.15526936831366No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC144211816.19481145874451No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG848000.9522937871252799No Hit
ATCGATCCGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCCT666830.7488420590433378No Hit
ATCGATCCGACTCCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT164560.18479889812421707No Hit
ATCGATCCGACTCCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA155420.17453478820166393No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTT134170.15067129412570615No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTA129320.1452248025366052No Hit
ATCGATCCGACTCCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG121330.13625212876404508No Hit
ATCGATCCGACTCCGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC115920.13017676391929534No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTC97660.10967100383331939No Hit
ATCGATCAGACTCGGTGCCACTTTTTCAACTAGTTCCGCTTACACAGCTG97470.10945763612158141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGATC8232950.073.942051
TCGATCC8076100.073.753262
CGATCCG8055400.073.672213
GATCCGA8113100.073.2069554
ATCCGAC8143300.072.844085
TCCGACT8149000.072.592866
GACTCGG8107450.072.275559
CGACTCG8059000.072.233068
ACTCGGT8133300.072.06393410
CCGACTC8222400.071.8675467
CTCGGTG8122600.071.82767511
TCGGTGC8131750.071.7359312
CGGTGCC8114300.071.67702513
GGTGCCA8114400.071.4764514
GACTCCG158150.071.3735669
GTGCCAC8275650.071.25416615
GCCACTT8280450.071.18918617
CCACTTT8319700.071.15616618
TGCCACT8317900.071.0261216
ACTCCGT154550.070.95425410
Produced by FastQC (version 0.11.5)